Quicking Biotech Co., Ltd.
Main products:boline brulella altest,salmonella test,cpv+ccv agtest
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  • Contact Person : Mr. Tang Spark
  • Company Name : Quicking Biotech Co., Ltd.
  • Tel : 86-21-50947188
  • Fax : 86-21-50947186
  • Address : Shanghai,Pudong,3F, Production Building, No. 1998, South Yanggao Road
  • Country/Region : China
  • Zip : 200125

Penicillin Rapid Test (Milk)

Penicillin Rapid Test (Milk)
Product Detailed
Quicking Penicillin Rapid Test has lower price but nearly the same quality compared to that made by European or USA.

Penicillin G Rapid Test (Milk)

Cat No.: W81046-1

1.        INTENDED USE

Quicking Penicillin G Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Penicillin G residue in milk.

Cut-off:   4 ppb

Assay Time:  5 – 10 min

Sample: Defatted milk

2.        PRINCIPLE OF THE ASSAY

Quicking Penicillin G Rapid Test is based on competitive lateral flow immunochromatographic assay. The Penicillin G conjugate in the test zone will capture the immuno-gold (colloid gold-Penicillin G antibody conjugate), when there is very little dissociative Penicillin G in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The test band (T zone) will be not visible if Penicillin G is present in concentration of 4 ppb and above which explains a positive result.

3.        KIT COMPONENT

-           10×foil pouches each contain one cassette and a desiccant

-           20×pipettes

-           10×centrifugal tubes (1.5 mL)

-           1×plastic canister containing 10 microwells and a desiccant

-           Product Manual

4.        TEST PROCEDURE

-           Bring 2-3 mL of the under tested milk to room temperature (20-30°C).

-           Collect 1mL of milk sample into a centrifugal tube. Do centrifugation at 7000 rpm for 3-4 min to separate the fat component.

-           Take out the microwells strip from the plastic canister. Take one well and tear off the film.

-           Insert the pipette through the fat layer (5 mm below) to collect the defatted milk towards the reticle in the pipette (200 μL) and transfer it into the well.

-           Use another pipette to repeatedly suck and extrude the sample until all red reagents are completely dissolved. Wait for about 2 min. NOTE: The sensitivity of the strip is positive correlated to the reaction time. It is suggested to wait for 1-2 min before running the assay.

-           Take out the cassette from the foil pouch and place it horizontally.

-           Suck the mixture in the well and gradually drip 3 drops into the assay sample hole “S”.

-           Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

5.        INTERPRETATION OF RESULTS

Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Penicillin G is at or above 4 ppb in the samples.

Negative: The presence of both clear band in C zone and T zone.

Invalid: No colored band appears in C zone.

6.        SPECIFICITY

The results are negative when the test card is applied to detect 100 ppm of Chloramphenicol, Aminoglycosides, Tetracyclines, Macrolides, Sulfonamides, Cephapirin and Ceftiofur; 200 ppb of  Amoxicillin; 50 ppb of Cloxacillin and 20 ppb of Ampicillin.

7.        STORAGE

The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

8.        PRECAUTIONS

-           For best results, please strictly adhere to these instructions.

-           All reagents must be at room temperature before running the assay.

-           Do not remove test cassette from its pouch until immediately before use.

-           Do not reuse the test kit.

-           Do not use the test beyond its expiration date marked on the foil pouch.

-           The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

9.        LIMITATION

Quicking Penicillin G Rapid Test is an useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. It provides a semi-quantitative method to detect the Penicillin G above 4 ppb in milk. If you want a quantitative result, it is suggested to apply other method such as ELISA / HPLC in practice.

Penicillin Rapid Test (Milk)



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